: Karyology, morphology and anatomy studies of medicinal plant Salvia nemorosa in the North East of Iran

ناشر: Entomology and Applied Science Letters

توضیحات: In this study, the karyotype, morphology and anatomy of the petiole and stem the herb Salvia nemorosa was discussed. After collecting and identifying plant , it conducted qualitative and quantitative traits, then, Very thin slices prepared and the samples were stained. To prepare a karyotype, three methods were used which method use of colchicine was more effective. S. nemorosa covered with soft short glandular trichome with low-density and dense non glandular trichome. The general shape of petiole, crescent-shaped, 5-bundle sheath of the center and 2 bundle sheath on either side there. Circular stem epidermis cells, Pith parenchyma cell with 40 to 50 layers of cells polygonal, karyological results showed that the chromosome number of S. nemorosa (2n = 14) with karyotype formula is 4 sm + 3m.

: Anatomy of the medicinal plants Salvia hydrangea, Salvia glutinosa and Salvia syriaca in iran

ناشر: journal of research in economy in international scientific research

توضیحات: Salvia is one of the most useful medicinal plants in Iran. Various species of Salvia are used in the industry for preparation of medicine. In this study, we compared the anatomy of leaves, petioles and a variety of glandular and non-glandular trichomes of three species of Salvia viz; Salvia hydrangea, Salvia glutinosa and Salvia syriaca present in Iran. Anatomy studies were performed by identification, cutting and staining the samples. The overall shape of leaves, petioles, number of layers and shape of chlorenchyma cells, scleroid the number of vascular bundles, stomata, mesophilic cells, number of layers of palisade parenchyma, alveolar parenchyma, other parenchyma cells, glandular and non-glandular cells and their counts were analysed and studied. In all three species, stomata type was found to be diacytic. The main vein reported in Salvia glutinosa and Salvia syriaca was square and isobilateral in Salvia hydrangea.

: Petiole anatomy Study of some medicinal species of Salvia L. in Iran

ناشر: Entomology and Applied Science Letters

توضیحات: In this study, the petiole anatomy of six species of Salvia the family Lamiaceae named Salvia staminia, S. limbata, S. viridis, S. multicalis, S. bractata, S. palastinia Discussed and compared. Considered samples were cutting and colored. The results showed that almost all species differences in terms of overall shape, shape and number of vascular bundles, trichomes type, number and shape of the epidermis, Collenchyma, parenchyma showed. None of the petioles have n , t peltate glandular trichomes. The highest number of Vascular bundles in the petiole S.limbata were observed. The most prominent square shape of petiole in S. Stamina and S. palastinia were reported The highest number of the parenchyma layers in species S. palestinia were observed.

: A robust method for identification and in-planta detection of Verticillium dahliae in the infected olive trees, using real-time PCR and nested PCR

ناشر: Physiological and Molecular Plant Pathology

توضیحات: Verticillium wilt is a major disease of olive (Olea europaea) orchards worldwide. To control the disease, use of a reliable method for pre-planting screening of new planting stocks for infection by V. dahliae is essential. In this study, 20 symptomatic and asymptomatic olive trees, sampled from Zahak region of Sistan in Iran, were analyzed with real-time and nested PCR for in planta detection of V. dahliae. We could confirm the presence of V. dahliae in 12 out of 20 (60%) sampled trees using real-time PCR, whereas nested PCR produced amplicons for all tested symptomatic and asymptomatic samples (100% of tested trees), indicating that nested PCR was more efficient method than real-time PCR for in planta detection of V. dahliae. We used the designed nested PCR to determine also the pathotype of V. dahliae using Vdf2/Vdr2 primer set at the second round, which could produce the 297-bp amplicon for all tested samples infected with non-defoliating pathotype of V. dahliae. We showed that although the Verticillium wilt is spread in the olive cultivation areas in Zahak region of Sistan, nested PCR can be deployed for the timely detection and thereby prevention of the further spread of V. dahliae in the olive cultivation areas.

: بررسی و شناسایی آلودگی ورتیسیلیومی در باغات زیتون شهرستان زهک با استفاده از تکنیک های nested pcrو real-time pcr

ناشر: اولین همایش ملی علوم کشاورزی و زیست محیطی ایران

توضیحات: پژمردگی ورتیسیلیومی ناشی از قارچ Verticillium dahliae یکی از مهم ترین بیماری های تهدید کننده زیتون در سراسر جهان، از جمله باغات زیتون در ایران است.با توجه به توسعه اخیر کشت زیتون در منطقه سیستان این پژوهش جهت بررسی انتشار این بیماری و آلودگی درختان زیتون در منطقه سیستان انجام گرفته است.بدین منظور نمونه برداری از 20 درخت مشکوک با داشتن علائمی مانند سرخشکیدگی و ریزش برگ، از بخش های مختلف باغات زیتون پژوهشکده کشاورزی دانشگاه زابل و مرکز تحقیقات کشاورزی زهک صورت گرفت. پس از استخراج DNA آنالیز Real-time PCR با استفاده از پرایمرهای ITS اختصاصی V. dahliae انجام شد. از تعداد 20 نمونه (درخت) تست شده که از ارقام زرد، روغنی و کرونایکی بودند تعداد 12 نمونه آلودگی به V. dahliae را نشان دادند. در ادامه با هدف تعیین پاتوتایپ عامل بیماری، از پرایمرهای اختصاصی ایزوله های V. dahliae نوع D و ND برای تست Nested PCR نمونه های DNA کلی استخراج شده از 12 نمونه ی برگی درختانی که آلودگی آن ها مورد تایید قرار گرفته بود استفاده شد. نتایج نشان داد پاتوتایپ عامل بیماری از نوع ND می باشد،در انتها برای راستی آزمایی نتایج، محصول واکنش زنجیره ای پلیمراز به شرکت Macrogen کره جنوبی ارسال و توالی یابی شد و با استفاده از نرم افزار بلاست در وب سایت NCBI- Blast Search درصد تشابه توالی ناحیه مورد مطالعه در V. dahliae با توالی های ثبت شده در پایگاه NCBI تعیین شد، که اکثریت توالی های ثبت شده در سطح حدود 98 درصد بیشترین تشابه را با توالی های بدست آمده داشتند که این نتایج بر این دلالت دارد که محصولات واکنش زنجیره ای پلیمراز به درستی تکثیر شده اند.